ASCP-MLT Exam Dumps : MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)

Polyspecific antihuman globulin (AHG) reagent contains both anti-IgG and anti-C3d.

Polyspecific antihuman globulin (AHG) reagent used in antiglobulin testing should react with which one of the following?

RAST tests, or Radioallergosorbent tests, are used to screen for an allergy to a specific substance or substances if a person presents with allergy-like symptoms.

The assay which is most helpful in identifying specific allergens is:

If your reactions are strong at immediate spin (3+) and then get weaker at AHG (w+), it could mean the presence of a strong cold antibody.

Cold antibodies tend to be IgM and their optimum phase for reactivity is immediate spin. Incubation and washing of the sample may cause the agglutination that occurred at room temperature to break down. This would appear as a weaker reaction at AHG.

If the reaction strengths varied in each panel cell then that could be an indication that there are multiple antibodies present.

Your screen cells are 3+ at immediate spin and weak (W)+ at AHG. Your auto control is negative for both phases. Some of your antibody panel cells are 3+ at immediate spin and negative at AHG. What should you suspect?